Molecular Screening of Exon 12 of Janus Kinase 2, Exon 9 of Calreticulin Genes in Polycythemia Vera Patients with Unmutated Janus Kinase 2 V617F

The calreticulin gene has nine exons and most of the frequent mutations screened observed in 9th exon myeloproliferative neoplasm patients, especially essential thrombocythemia primary myelofibrosis. In current study, an uncommon mutation was a 51 y old man with polycythemia vera phenotype. Somatic novel homozygous affect 9 gene. These recent genetic variations were not reported previous studies. Molecular screening may be important for patients that are negative Janus kinase 2 V617F 12 2. A total 11 previously diagnosed by amplification refractory system-polymerase chain reaction technique. Sanger sequencing performed to screen last addition, all wild lesion neoplasms as second biomarker, particularly thromobocythemia myelofibrosis, is more informative diagnosis. Molecularly, phenotypes, genotyping useful.